RBPs are key key drivers of gene expression by controlling RNA fate. However, our knowledge about RBPs in plants is very limited. I our recent work, we report an improved RNA interactome capture approach to discover RNA-binding proteins (RBPs) in plant leaves . Using this ‘plant-adapted RNA interactome capture’ (ptRIC) we have identified hundreds novel RBPs, including many enzymes and proteins from the photosynthetic apparatus. ptRIC did not only allowed the generation of the deepest ‘RBPome’ of plant tissue to date, but also opens the possibility to study how the RBPome remodels in response to environmental, physiological and pathological cues.
This work is an interdisciplinary collaborative effort between the Castello lab (Department of Biochemistry) and Preston lab (Department of Plant Sciences) at the University of Oxford. Find out more about this work here:
Discovering the RNA-binding proteome of plant leaves with an improved RNA interactome capture method. Marcel Bach-Pages, Felix Homma, Jiorgos Kourelis, Farnusch Kaschani, Shabaz Mohammed, Markus Kaiser, Renier A. L. van der Hoorn, Alfredo Castello*, Gail M. Preston*