Part II, SCORE and Princeton students

Eve Smith, part II student 2015-2016

She investigated how a complex DNA virus as vaccinia alters the cellular RNA-bound proteome. Amongst other things she discovered that vaccinia virus encodes more than two dozens of RNA-binding proteins. Eve was then accepted as PhD student in Imperial College.

Thomas Davis, part II student 2016-2017

He studied the protein-protein interactome of GEMIN5 in sindbis virus infected cells. He discovered that GEMIN5 oligomerise and interact with the 60S ribosomal subunit to regulate translation of viral RNAs. His work was published in Garcia-Moreno et al., Mol Cell 2019. Tom then move to work as consultant.

Matt Avedson, SCORE programme, U. Wisconsin 2017

He developed the first HIV construct expressing a tagged version of Rev. He showed that this virus replicates as wild type and his work paved the way to following projects aiming to understand the role of Rev in infection.

Victoria Cox, part II student 2017-2018

She was interested in understanding the role of cellular RNA-binding proteins in sindbis virus infection. She carried out a targeted genetic screen to determine if the RNA-binding proteins identified in our proteomic studies were important for sindbis infection. She identified several antiviral and proviral RNA-binding proteins and her work was included in Garcia-Moreno et al., Mol Cell 2019. She was then accepted as PhD student in Imperial College.

Miguel Aracena, part II student 2017-2018

He was interested in the protein Rev of HIV and how it regulates RNA metabolism in the infected cell. His project focused on elucidating the protein-protein interactions of this protein when expressed in a ‘real’ infection. His work is currently followed up by a PhD student and Miguel got a position in Oxford Nanopore Technologies.

Thomas Anderson, SCORE programme, U. Wisconsin 2018

He continued the work with vaccinia virus aiming to understand which viral proteins interact with RNA and why.

Leslie Chan, Princeton student 2018-2019

Leslie worked in the Castello lab for five months as part of her degree in Princeton. She was interested in the understanding the role of a family of tumour suppressors recently classified as RNA-binding proteins by RNA interactome studies.

Jessica Quirke, Part II Biochemistry student

I aim to visualise cellular RNA-binding proteins incorporated into HIV capsids using single particle tracking and super-resolution microscopy.

Morgan Elsmore, Part II Biochemistry student

My goal is to develop a robust and sensitive method to capture viral RNA with the proteins bound to it. In the long term, this method will be used to decipher the composition of viral RBPs.